Selective Fluorimetric Detection of Pyrimidine Nucleotides in Neutral Aqueous Solution with a Styrylpyridine-Based Cyclophane by Julika Schlosser Julian F. M. Hebborn Daria V. Berdnikova & Heiko Ihmels
Author:Julika Schlosser, Julian F. M. Hebborn, Daria V. Berdnikova & Heiko Ihmels
Format: pdf
Tags: A styrylpyridine-containing cyclophane with diethylenetriamine linkers is presented as a host system whose association with representative nucleotides was examined with photometric and fluorimetric titrations. The spectrometric titrations revealed the formation of 1:1 complexes with log Kb values in the range of 2.3–3.2 for pyrimidine nucleotides TMP (thymidine monophosphate), TTP (thymidine triphosphate) and CMP (cytidine monophosphate) and 3.8–5.0 for purine nucleotides AMP (adenosine monophosphate), ATP (adenosine triphosphate), and dGMP (deoxyguanosine monophosphate). Notably, in a neutral buffer solution, the fluorimetric response to the complex formation depends on the type of nucleotide. Hence, quenching of the already weak fluorescence was observed with the purine bases, whereas the association of the cyclophane with pyrimidine bases TMP, TTP, and CMP resulted in a significant fluorescence light-up effect. Thus, it was demonstrated that the styrylpyridine unit is a useful and complementary fluorophore for the development of selective nucleotide-targeting fluorescent probes based on alkylamine-linked cyclophanes., fluorescent dyes; nucleotide recognition; heterocycles; host–guest chemistry
Download
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.
Administration | Assessment |
Educational Psychology | Experimental Methods |
History | Language Experience Approach |
Philosophy & Social Aspects | Reform & Policy |
Research |
The Art of Coaching Workbook by Elena Aguilar(48569)
Trainspotting by Irvine Welsh(20139)
Twilight of the Idols With the Antichrist and Ecce Homo by Friedrich Nietzsche(17752)
Fangirl by Rainbow Rowell(7892)
Periodization Training for Sports by Tudor Bompa(7375)
Change Your Questions, Change Your Life by Marilee Adams(6702)
This Is How You Lose Her by Junot Diaz(5844)
Grit by Angela Duckworth(4776)
Red Sparrow by Jason Matthews(4733)
Asking the Right Questions: A Guide to Critical Thinking by M. Neil Browne & Stuart M. Keeley(4640)
Paper Towns by Green John(4208)
Room 212 by Kate Stewart(4150)
Ken Follett - World without end by Ken Follett(4001)
The Sports Rules Book by Human Kinetics(3628)
Housekeeping by Marilynne Robinson(3454)
The Motorcycle Diaries by Ernesto Che Guevara(3376)
Exercise Technique Manual for Resistance Training by National Strength & Conditioning Association(3329)
Introduction to Kinesiology by Shirl J. Hoffman(3327)
Papillon (English) by Henri Charrière(3320)